Clark, D;
Moore, CM;
Flanagan, M;
Van Bocxlaer, K;
Piperaki, E-T;
Yardley, V;
Croft, SL;
Tyson, J;
Whitehouse, SP;
O'Halloran, J;
et al.
Clark, D; Moore, CM; Flanagan, M; Van Bocxlaer, K; Piperaki, E-T; Yardley, V; Croft, SL; Tyson, J; Whitehouse, SP; O'Halloran, J; Krishna, S; Staines, HM
(2019)
An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture.
BIOTECHNIQUES.
ISSN 0736-6205
(In Press)
SGUL Authors: Staines, Henry Michael Moore, Catherine Margaret Clark, David John
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Abstract
The aim of this study was to assess pathogen DNA extraction with a new spin column-based 3method (DNA-XT). DNA from i) whole blood samples spiked with Plasmodium falciparum 4or ii) Leishmania donovani amastigote culture was extracted with DNA-XT and compared 5with that produced by a commercial extraction kit (DNeasy). Eluates from large and small 6sample volumes were assessed by PCR and spectroscopy. Using a small volume (5 μl) of 7blood, the DNA-XT and DNeasy methods produced eluates with similar DNA 8concentrations, 0.63 versus 1.06 ng/μl, respectively. The DNA-XT method produced DNA 9with lower PCR inhibition than DNeasy. The new technique was also twice as fast and 10required fewer plastics and manipulations but had reduced total recovered DNA compared 11with DNeasy.
| Item Type: | Article | ||||||||||||
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| Keywords: | 06 Biological Sciences, 10 Technology, Bioinformatics | ||||||||||||
| SGUL Research Institute / Research Centre: | Academic Structure > Infection and Immunity Research Institute (INII) | ||||||||||||
| Journal or Publication Title: | BIOTECHNIQUES | ||||||||||||
| ISSN: | 0736-6205 | ||||||||||||
| Dates: |
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| Publisher License: | Publisher's own licence | ||||||||||||
| Projects: |
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| URI: | http://sgultest.da.ulcc.ac.uk/id/eprint/111368 |
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