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Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy

Jonas, KC; Hanyaloglu, AC (2019) Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy. In: G Protein-Coupled Receptor Signaling: Methods and Protocols. Methods in Molecular Biology (1947). (ed Tiberi, M.) Humana Press (Springer Imprint), pp. 337-348. DOI: https://doi.org/10.1007/978-1-4939-9121-1
SGUL Authors: Jonas, Kim Carol

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Abstract

Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane.

Item Type: Book Section
Additional Information: This is a post-peer-review, pre-copyedit version of an article published in G Protein-Coupled Receptor Signaling: Methods and Protocols. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9121-1_19
Keywords: Developmental Biology, 0601 Biochemistry And Cell Biology
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Methods in Molecular Biology
Editors: Tiberi, M
ISBN: 978-1-4939-9120-4, 978-1-4939-9121-1, 1064-3745, 1940-6029
ISSN: 1940-6029
Series Name: Methods in Molecular Biology
Dates:
DateEvent
11 April 2019Published
11 August 2018Accepted
Publisher License: Publisher's own licence
Projects:
Project IDFunderFunder ID
UNSPECIFIEDBiotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDSociety for Endocrinologyhttp://dx.doi.org/10.13039/501100000382
URI: http://sgultest.da.ulcc.ac.uk/id/eprint/110043
Publisher's version: https://doi.org/10.1007/978-1-4939-9121-1

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