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Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia.

Wallace, AE; Whitley, GS; Thilaganathan, B; Cartwright, JE (2015) Decidual natural killer cell receptor expression is altered in pregnancies with impaired vascular remodeling and a higher risk of pre-eclampsia. J Leukoc Biol, 97 (1). pp. 79-86. ISSN 1938-3673 https://doi.org/10.1189/jlb.2A0614-282R
SGUL Authors: Cartwright, Judith Eleanor Whitley, Guy St John Thilaganathan, Baskaran

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Abstract

During pregnancy, a specialized type of NK cell accumulates in the lining of the uterus (decidua) and interacts with semiallogeneic fetal trophoblast cells. dNK cells are functionally and phenotypically distinct from PB NK and are implicated in regulation of trophoblast transformation of the uterine spiral arteries, which if inadequately performed, can result in pregnancy disorders. Here, we have used uterine artery Doppler RI in the first trimester of pregnancy as a proxy measure of the extent of transformation of the spiral arteries to identify pregnancies with a high RI, indicative of impaired spiral artery remodeling. We have used flow cytometry to examine dNK cells isolated from these pregnancies compared with those from pregnancies with a normal RI. We report a reduction in the proportion of dNK cells from high RI pregnancies expressing KIR2DL/S1,3,5 and LILRB1, receptors for HLA-C and HLA-G on trophoblast. Decreased LILRB1 expression in the decidua was examined by receptor blocking in trophoblast coculture and altered dNK expression of the cytokines CXCL10 and TNF-α, which regulate trophoblast behavior. These results indicate that dNK cells from high RI pregnancies may display altered interactions with trophoblast via decreased expression of HLA-binding cell-surface receptors, impacting on successful transformation of the uterus for pregnancy.

Item Type: Article
Additional Information: This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 international (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: KIR, LILRB1, chemokine, trophoblast, Decidua, Female, Flow Cytometry, Humans, Pre-Eclampsia, Pregnancy, Receptors, Natural Killer Cell, Reverse Transcriptase Polymerase Chain Reaction, Risk Factors, Ultrasonography, Doppler, Uterine Artery, Vascular Remodeling, trophoblast, LILRB1, KIR, chemokine, Humans, Pre-Eclampsia, Reverse Transcriptase Polymerase Chain Reaction, Flow Cytometry, Uterine Artery, Ultrasonography, Doppler, Receptors, Natural Killer Cell, Pregnancy, Decidua, Risk Factors, Female, Vascular Remodeling, Immunology, 1107 Immunology
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Vascular (INCCVA)
Journal or Publication Title: J Leukoc Biol
ISSN: 1938-3673
Language: eng
Dates:
DateEvent
January 2015Published
7 November 2014Published Online
11 September 2014Accepted
Projects:
Project IDFunderFunder ID
091550Wellcome TrustUNSPECIFIED
PubMed ID: 25381387
Web of Science ID: WOS:000347463400011
Go to PubMed abstract
URI: http://sgultest.da.ulcc.ac.uk/id/eprint/107405
Publisher's version: https://doi.org/10.1189/jlb.2A0614-282R

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