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Differences in collagen ultrastructure of human first trimester decidua basalis and parietalis: Implications for trophoblastic invasion of the placental bed

Sinai Talaulikar, V; Kronenberger, K; Bax, BE; Moss, R; Manyonda, I (2014) Differences in collagen ultrastructure of human first trimester decidua basalis and parietalis: Implications for trophoblastic invasion of the placental bed. JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 40 (1). 80 - 88. ISSN 1341-8076 https://doi.org/10.1111/jog.12127
SGUL Authors: Bax, Bridget Elizabeth Manyonda, Isaac Tainzana

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Abstract

AIM: The human embryo-maternal interface in the first trimester of pregnancy is an area of extensive tissue remodeling. Because collagen is the most abundant constituent of the extracellular matrix of the placental bed, successful invasion must involve its rapid turnover. We compared the nature and distribution of collagen fibrils in decidua basalis and parietalis. METHODS: We used a direct-vision hysteroscopic technique to obtain biopsies of the decidua basalis and parietalis from 11 women undergoing pregnancy termination in the first trimester. The biopsies were subjected to light, transmission and scanning electron microscopy, and immunohistochemical studies using mouse monoclonal antibodies against cytokeratin 7 and collagen types I, III and V. RESULTS: Collagen fibrils in the stroma of decidua basalis were significantly thicker when compared to those in decidua parietalis (56.48 ± 1.37 nm vs 45.64 ± 0.85 nm; P < 0.0001 [mean ± standard error]) between 9 and 12 weeks gestation, but this difference in thickness was not observed at gestations below 9 weeks. In basalis, the fibrils appeared disrupted at most places surrounding the decidual/trophoblast cells while a uniform regular arrangement was preserved throughout most of parietalis. CONCLUSION: There are differences in the ultrastructure of collagen fibrils between basalis and parietalis, with thicker and disrupted fibrils within abundant amorphous tissue in basalis, and thinner uniform fibrils in parietalis. These differences may reflect an adaptive response by decidua or a direct consequence of the invading trophoblast cells.

Item Type: Article
Additional Information: This is the accepted version of the following article: Sinai Talaulikar, V., Kronenberger, K., Bax, B. E., Moss, R. and Manyonda, I. (2014), Differences in collagen ultrastructure of human first trimester decidua basalis and parietalis: Implications for trophoblastic invasion of the placental bed. Journal of Obstetrics and Gynaecology Research, 40: 80–88. doi: 10.1111/jog.12127 which has been published in final form at http://dx.doi.org/10.1111/jog.12127.
Keywords: basalis, collagen, decidua, extracellular matrix, parietalis, Science & Technology, Life Sciences & Biomedicine, Obstetrics & Gynecology, OBSTETRICS & GYNECOLOGY, EXTRACELLULAR-MATRIX, EXTRAVILLOUS TROPHOBLAST, MOUSE ENDOMETRIUM, FIBRILS, DECIDUALIZATION, TISSUE, CELLS, Obstetrics & Reproductive Medicine, 1114 Paediatrics And Reproductive Medicine
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Vascular (INCCVA)
Journal or Publication Title: JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
ISSN: 1341-8076
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Dates:
DateEvent
1 January 2014Published
Web of Science ID: WOS:000329141700012
URI: http://sgultest.da.ulcc.ac.uk/id/eprint/107120
Publisher's version: https://doi.org/10.1111/jog.12127

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